The Night Tour Refuge is a 2-hour light hike in search of the creatures of the night, recommended for anyone who wants to see wildlife such as sloths, armadillos, porcupines, opossums, coatis, olingos, and kinkajous, as well as sleeping birds and a wide variety of insects, among others.

El Refugio is a privileged place to see bird species such as sleeping toucans, mot-mots, and black guans; treetop animals such as monkeys, coatis, olingos, sloths, frogs, snakes, and a wide variety of insects. On a typical tour, you will see 3-5 species of mammals, 5–10 species of birds, a couple of snakes or frogs, and many types of insects. This is the widest variety of animals you will see on any other night tour in the area.

You will join a group of four to six people with a local tour guide, who will give you an introduction and flashlights before starting the walk through the trails of El Refugio. Places are limited, so you must reserve in advance.